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KMID : 0903619970380010039
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Journal of the Korean Society for Horticultural Science
1997 Volume.38 No. 1 p.39 ~ p.42
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Random Amplified Polymorphic DNA Analysis of Capsicum germplasm
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김기택/Kim, Ki Taek
강병철/김도선/오대근/Kang, Byoung Cheorl/Kim, Do Sun/Oh, Dae Guen
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Abstract
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To evaluate genetic distance among 43 pepper accessions of diverse geographic origins, we have used RAPDs (random amplified polymorphic DNAs). The number of fragments per primer ranged between 4 and 17, with an average of 10 per primer. The size of the amplified fragments ranged between 200 and 4,000 base pairs. Overall, fifty-nine polymorphic fragments were generated by 35 primers. While a considerable degree of polymorphism was detected among species, but within the species, C. annuurn, a much lower degree of polymorphism was detected. C. aunuum, C. chinense, and C. pubescens were more closely related to one another than to the species, C. baccutum. UMPGA separates most Capsicum annum peppers into two main groups. Surprisingly three genotypes; Namjin, Hatvani and 3-25-27 were distinct from the other genotypes of C. annuum studied.
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